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OBTENCIÓN DE GARRAPATAS DERMACENTOR NITENS (NEUMANN, 1897) (ACARI: IXODIDAE) EN BOVINOS
Alier Fuentes Castillo
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POBLACIONES NATURALES DE NEMATODOS ENTOMOPATÓGENOS PRESENTES EN SUELOS DE SIETE ESTADOS DE VENEZUELA (Natural populations of entomopathogenic nematodes present in soils of seven Venezuelan States)
Mayra G. Rodríguez Hernández
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TOMATO LEAF MINER: RESISTENCE CULTIVARS AND BIOCONTROL AGENTS AS ALTERNATIVES TO FACE THE IMPACT OF ITS DAMAGE IN CUBA
Leticia Duarte Martínez
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META-ANÁLISIS DE LAS ESTRATEGIAS DE MANEJO DE TISANÓPTEROS QUE AFECTAN AL CULTIVO DE LA PAPA (Meta –analysis of management strategies for trips that affect potato crosp)
Lizandra Guerra-Arzuaga
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ENTOMOFAUNA ASOCIADA A S. FRUGIPERDA EN EL CULTIVO DEL MAÍZ (ZEA MAYS L) EN ÉPOCA NO LLUVIOSA (Entomofauna associated with S. frugiperda in the corn (Zea mays L) crop in the non-rainy season)
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VERIFICACIÓN CONTINUA DEL PROCESO DE FABRICACIÓN DEL INGREDIENTE FARMACÉUTICO ACTIVO DE SURFACEN®
Wilma Alfonso
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INNOVACIÓN TECNOLÓGICA EN EL DESARROLLO INDUSTRIAL DE BIOPRODUCTOS A PARTIR DEL CAPULLO DE Bombyx mori
Hector Correa Rivero
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Estudio de parametros hematologicos y bioquimicos de cerdos en ceba suplementados con ractopamina
Yaneris Cabrera Otaño
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INNOVACIÓN EN LA INSTALACIÓN Y PUESTA EN MARCHA DE UNA PLANTA PARA LA PRODUCCIÓN DE AGUA PURIFICADA.
Adrian García Pedroso
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CASAS DE CULTIVO AUTOMATIZADAS: LA EFICIENCIA AL ALCANCE DE NUESTROS SUELOS.
Miguel Eduardo Borrego Corona
Evento IV Seminario Internacional de Sanidad Agropecuaria
starts on
8 de mayo de 2023, 8:00:00 -0400
MOLECULAR BASIS OF PHARMACORRESISTANCE TO BENZIMIDAZOLS IN β-TUBULIN MUTANTS OF CYATHOSTOMINS
(1 hora 30 minutos)
Dany Naranjo Feliciano
Researcher
at Centro Nacional de Sanidad Agropecuaria
danynaranjofeliciano@gmail.com
Cyathostomins are highly prevalent equine gastrointestinal parasites. Benzimidazole-2-carbamate derivatives, such as albendazol and oxibendazol, are the most commonly used drugs for the control and treatment in Cuba. These antiparasitics selectively bind to the β-tubulin monomer and inhibit microtubule polymerization. However β-tubulin mutations can convert susceptible wild type worms to unsusceptible, thereby enabling benzimidazole resistance. Moreover, the relation between the mechanism of action and the binding site of bezimidazole has not yet been explained accurately. By using the β-tubulin gene DNA fragment sequence - Cylicostephanus goldi (wild type control, not determined) - and five cyathostomin species (Cyathostomum catinatum, Cyathostomum pateratum, Cylicostephanus longibursatus, Cylicostephanus minutus, Cylicocyclus nassatus) pre-selected by extra-label use of albendazole and resistant to oxibendazole, were employed as initial biological material. By applying homology modeling techniques, we were able to build a 3D structure of the β-tubuline protein, generated using the structure of the mammal Ovis aries, considered as a low susceptible organism, as a template. In order to identify at the molecular level, of the mode of action, molecular docking and molecular dynamics calculations were carried out with these models. The results were in good agreement with the most common amino acid mutations associated with drug resistance (with single -F167Y- and doubly mutant -S165C, F167Y-) in the colchicine binding site to β-tubulin. This study provided insight into the structural composition of β-tubulin and microtubule in cyathostomins and possible mechanisms of benzimidazole resistance within the study population.