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BIODIVERSIDAD FÚNGICA, PERSPECTIVAS DE SUS APLICACIONES Y SU REPERCUSIÓN EN LA SOSTENIBILIDAD DE LA SOCIEDAD CUBANA.
Beatriz Ramos García
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CASAS DE CULTIVO AUTOMATIZADAS: LA EFICIENCIA AL ALCANCE DE NUESTROS SUELOS.
Miguel Eduardo Borrego Corona
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RESULTADOS DEL PROYECTO BIOC EN EL DEPARTAMENTO DE CONSERVACIÓN BARACOA DEL PARQUE NACIONAL ALEJANDRO DE HUMBOLDT, CUBA
Norvis Hernández Hernández
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ESTRATEGIA DE GESTIÓN DE LOS RESIDUOS QUÍMICOS Y BIOLÓGICOS DE LA PRODUCCIÓN DE SURFACEN®
Marelis Hernández Fernández
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Estudio de parametros hematologicos y bioquimicos de cerdos en ceba suplementados con ractopamina
Yaneris Cabrera Otaño
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TRIPS (THYSANOPTERA: THRIPIDAE) DE INTERÉS AGRÍCOLA EN LA PROVINCIA DE SANCTI SPÍRITUS, CUBA CENTRAL
José Manuel Ramos Hernández
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Clasificación taxonómica de aislados de Trichoderma provenientes de la rizozfera de Theobroma cacao L. en la localidad de Baracoa
Acela Díaz de la Osa
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ACTIVIDAD ANTAGONISTA DE CEPAS FÚNGICAS SOBRE HONGOS PATÓGENOS DEL FRIJOL COMÚN (PHASEOLUS VULGARIS L.)
Yarelis Ortiz Núñez
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Determinación de N inorgánico del suelo. Disponibilidad de este nutriente en los sistemas suelo-planta-agua
Graciela Dueñas Vega
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INFLUENCIA DEL RECUBRIMIENTO DEL TECHO DE UN CENTRO DE PROCESAMIENTO DE SEMEN PORCINO COMO NORMA DE BIENESTAR ANIMAL EN LOS INDICADORES DE CALIDAD ESPERMÁTICA
Abelardo Núñez Velázquez
Evento IV Seminario Internacional de Sanidad Agropecuaria
starts on
8 de mayo de 2023, 8:00:00 -0400
MOLECULAR BASIS OF PHARMACORRESISTANCE TO BENZIMIDAZOLS IN β-TUBULIN MUTANTS OF CYATHOSTOMINS
(1 hora 30 minutos)
Dany Naranjo Feliciano
Researcher
at Centro Nacional de Sanidad Agropecuaria
danynaranjofeliciano@gmail.com
Cyathostomins are highly prevalent equine gastrointestinal parasites. Benzimidazole-2-carbamate derivatives, such as albendazol and oxibendazol, are the most commonly used drugs for the control and treatment in Cuba. These antiparasitics selectively bind to the β-tubulin monomer and inhibit microtubule polymerization. However β-tubulin mutations can convert susceptible wild type worms to unsusceptible, thereby enabling benzimidazole resistance. Moreover, the relation between the mechanism of action and the binding site of bezimidazole has not yet been explained accurately. By using the β-tubulin gene DNA fragment sequence - Cylicostephanus goldi (wild type control, not determined) - and five cyathostomin species (Cyathostomum catinatum, Cyathostomum pateratum, Cylicostephanus longibursatus, Cylicostephanus minutus, Cylicocyclus nassatus) pre-selected by extra-label use of albendazole and resistant to oxibendazole, were employed as initial biological material. By applying homology modeling techniques, we were able to build a 3D structure of the β-tubuline protein, generated using the structure of the mammal Ovis aries, considered as a low susceptible organism, as a template. In order to identify at the molecular level, of the mode of action, molecular docking and molecular dynamics calculations were carried out with these models. The results were in good agreement with the most common amino acid mutations associated with drug resistance (with single -F167Y- and doubly mutant -S165C, F167Y-) in the colchicine binding site to β-tubulin. This study provided insight into the structural composition of β-tubulin and microtubule in cyathostomins and possible mechanisms of benzimidazole resistance within the study population.