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GESTIÓN SEGURA DE LOS DESECHOS PELIGROSOS Y PRODUCTOS QUÍMICOS CADUCOS Y OCIOSOS
Maite Lorenzo Hernández
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CRECIMIENTO Y COMPOSICIÓN CORPORAL DE CERDOS CRIOLLO CUBANOS EN CEBA EXTENSIVA CON PALMICHE
Isabel Marta Santana Martinez
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POTENCIAL DE PRODUCCIÓN DE BIOGÁS DE LA VINAZA EN CODIGESTIÓN CON EXCRETAS PORCINAS.
José Luis de la Fuente Arzola
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PRESENCIA DE HIDROCARBURO AROMÁTICOS POLICÍCLICOS (HAPS) EN ESLABONES PRIMARIOS DE LA CADENA PRODUCTIVA DE LA LECHE EN LA REGIÓN OCCIDENTAL DE CUBA./ Presence of polyciclic aromatic (HAPs) in the milk production chain in the western region of Cuba.
Arturo Escobar Medina
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SISTEMA PARA GESTION DE MUESTRAS DE MICOPLASMAS.
Alvaro Betancourt Batista
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ESTUDIO DE COSTRAS BIOLÓGICAS DE SUELO Y SU EFECTO COMO BIOINDICADORES DE PROCESOS DE DEGRADACIÓN DE SUELO EN LA CPA MÁRTIRES DE BARBADOS DE LA PROVINCIA DE CIENFUEGOS, CUBA
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VERIFICACIÓN CONTINUA DEL PROCESO DE FABRICACIÓN DEL INGREDIENTE FARMACÉUTICO ACTIVO DE SURFACEN®
Wilma Alfonso
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PERFIL BIOQUÍMICO HEMATOLÓGICO DE NOVILLAS EN EMPRESAS GENÉTICAS DEL OCCIDENTE DE CUBA.
Malena Gámez González
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VALIDACIÓN DE MÉTODOS ANALÍTICOS PARA LA CUANTIFICACIÓN DE METABOLITOS EN LAS HOJAS DE MORERA (Morus alba L.).
Sirley González Laime
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EFFECT OF THE ESSENTIAL OIL OF Lippia graveolens KUNTH (MEXICAN OREGANO) ON Salmonella Typhimurium BIOFILM (Efecto del aceite esencial de Lippia graveolens Kunth (orégano mexicano) sobre la biopelícula de Salmonella Typhimurium)
Annie Rubio Ortega
Evento IV Seminario Internacional de Sanidad Agropecuaria
starts on
8 de mayo de 2023, 8:00:00 -0400
Estimating evolutionary changes between highly passaged and original parental lumpy skin disease virus strains
(1 hora 30 minutos)
Research into the phylogenetic relationships of lumpy skin disease virus (LSDV) strains was long overlooked, partially due to its original restricted distribution to sub-Saharan Africa. However, recent incursions into northern latitudes, and a rapid spread causing major economic losses worldwide, have intensified additional research on the disease and the causative virus. This study delineates the phylogeny of LSDV in the context of full genome sequences of strains recovered in the field, as well as strains highly passaged in cell culture. We sequenced the oldest known field strain to date (isolate LSDV/Haden/RSA/1954 [South Africa] recovered from an outbreak in 1954), a recent field isolate (LSDV/280-KZN/RSA/2018 [South Africa] sequenced directly from blood during an outbreak in 2018) and strain LSDV/Russia/Dagestan-75 (a high-passaged cell culture strain derived from the field strain, LSDV/Russia/Dagestan/2015 [Russia]). Sequence analysis placed the field strain LSDV/Haden/RSA/1954 in the same cluster (cluster 1.1) with attenuated Neethling-type commercial vaccine viruses, with eight SNP differences, discrediting the previously held hypothesis that cluster 1.1 vaccine strains were derived from cluster 1.2 field viruses via the process of attenuation between them. In contrast, the recent LSDV/280-KZN/RSA/2018 isolate grouped with other recent field isolates in cluster 1.2, providing evidence that cluster 1.1 strains were displaced by cluster 1.2 strains in South Africa. Based on the field isolates between 1954 and 2018, the substitution rate of 7.4 × 10-6 substitutions/site/year was established, with mutations occurring in either synonymous sites or intergenic regions. This is the first evolutionary metric recorded for LSDV. Comparing the genome sequences of high-passage strains of LSDV showed that propagation in vitro without animal host selective pressure generates mainly non-synonymous SNPs in virus-replication genes. These results improve our understanding of LSDV evolution and demonstrate that the population dynamics of circulating isolates is not constant, with LSDV associated with different genetic clusters dominating the landscape during specific periods in time.