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ESTUDIO DE PRODUCTOS NATURALES PARA USO ETNOMÉDICO Y COMO FUENTE PARA LA INVESTIGACIÓN Y EL DESARROLLO DE MEDICAMENTOS PARA ANIMALES Y HUMANOS
Luz María Sanchez Perera
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VALIDACIÓN DE MÉTODOS ANALÍTICOS PARA LA CUANTIFICACIÓN DE METABOLITOS EN LAS HOJAS DE MORERA (Morus alba L.).
Sirley González Laime
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Actividad antifúngica in vitro de nanopartículas de plata a partir de hojas de Leea coccinea frente a Fusarium spp. y Penicillium spp.
Yanisia Duarte Leal
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Potencial antagonista de cepas de la clase Bacilli aisladas de cultivares cubanos de trigo (Triticum aestivum L.)
Marcia M. Rojas Badía
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Características para la promoción del crecimiento de cepas de la clase Bacilli aisladas de cultivares cubanos de trigo (Triticum aestivum L.)
Alexander Govin Sanjudo
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Monitoreo de esporas fúngicas en el aire de cultivares de trigo durante dos meses del cultivo
Reynier Cruz Santana
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MODELO DE GESTIÓN PARA LA CIENCIA-TECNOLOGÍA E INNOVACIÓN EMPRESARIAL: ESTUDIO DE CASO EMPRESA LABIOFAM SANCTI SPÍRITUS
Rosaida Pérez López
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LEIFSONIA XYLI SUBSP. XYLI EN PLANTACIONES DE CAÑA DE AZÚCAR EN LA EMPRESA AZUCARERA MANUEL FAJARDO RIVERO
Alejandro Hernández Rubiella
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PRESENCIA DE HIDROCARBURO AROMÁTICOS POLICÍCLICOS (HAPS) EN ESLABONES PRIMARIOS DE LA CADENA PRODUCTIVA DE LA LECHE EN LA REGIÓN OCCIDENTAL DE CUBA./ Presence of polyciclic aromatic (HAPs) in the milk production chain in the western region of Cuba.
Arturo Escobar Medina
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IMPORTANCIA DE LA EXTRACCIÓN Y CARACTERIZACIÓN DE SEDIMENTO DE LOS EMBALSES Y SU USO EN LA PRODUCCIÓN ECOLÓGICA Y DIVERSIFICADA DE HORTALIZAS
Teresa Ambrosina Fraser Gálves
Evento IV Seminario Internacional de Sanidad Agropecuaria
starts on
8 de mayo de 2023, 8:00:00 -0400
Estimating evolutionary changes between highly passaged and original parental lumpy skin disease virus strains
(1 hora 30 minutos)
Research into the phylogenetic relationships of lumpy skin disease virus (LSDV) strains was long overlooked, partially due to its original restricted distribution to sub-Saharan Africa. However, recent incursions into northern latitudes, and a rapid spread causing major economic losses worldwide, have intensified additional research on the disease and the causative virus. This study delineates the phylogeny of LSDV in the context of full genome sequences of strains recovered in the field, as well as strains highly passaged in cell culture. We sequenced the oldest known field strain to date (isolate LSDV/Haden/RSA/1954 [South Africa] recovered from an outbreak in 1954), a recent field isolate (LSDV/280-KZN/RSA/2018 [South Africa] sequenced directly from blood during an outbreak in 2018) and strain LSDV/Russia/Dagestan-75 (a high-passaged cell culture strain derived from the field strain, LSDV/Russia/Dagestan/2015 [Russia]). Sequence analysis placed the field strain LSDV/Haden/RSA/1954 in the same cluster (cluster 1.1) with attenuated Neethling-type commercial vaccine viruses, with eight SNP differences, discrediting the previously held hypothesis that cluster 1.1 vaccine strains were derived from cluster 1.2 field viruses via the process of attenuation between them. In contrast, the recent LSDV/280-KZN/RSA/2018 isolate grouped with other recent field isolates in cluster 1.2, providing evidence that cluster 1.1 strains were displaced by cluster 1.2 strains in South Africa. Based on the field isolates between 1954 and 2018, the substitution rate of 7.4 × 10-6 substitutions/site/year was established, with mutations occurring in either synonymous sites or intergenic regions. This is the first evolutionary metric recorded for LSDV. Comparing the genome sequences of high-passage strains of LSDV showed that propagation in vitro without animal host selective pressure generates mainly non-synonymous SNPs in virus-replication genes. These results improve our understanding of LSDV evolution and demonstrate that the population dynamics of circulating isolates is not constant, with LSDV associated with different genetic clusters dominating the landscape during specific periods in time.