The plants of Lippia graveolens Kunth (sin: L. berlandieri Schauer) have been used for therapeutic of respiratory and digestive conditions. The essential oil of plants grown in Cuba has a powerful antibacterial activity on Salmonella enterica. The aim of study was evaluating the activity of the essential oil of Lippia graveolens Kunth plants grown in Cuba on Salmonella Typhimurium biofilm. The inhibition effect of this essential oil on biofilm formation by Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028 was determined by biomass reduction with crystal violet, and the minimum biofilm inhibitory concentration (MBIC) was identified by plate counts. Swimming and swarming assays were used to evaluate the effect on motility structures. Also, the effects on preformed biofilms and minimum biofilm eradication concentration (MBEC) were determined. The essential oil of L. graveolens inhibits the formation of biofilms of S. Typhimurium and destroys preformed biofilms without generating persistent cells. MBIC values of the essential oil of L. graveolens coincided with those of MBEC at 0,5 mg/mL. Concentrations lower than MBIC, L. graveolens essences does not affect the bacterial swimming and swarming movements. The study revealed the inhibitory effect of L. graveolens essential oil on S. Typhimurium biofilm, and it show a natural promising candidate for disinfection of this pathogen.